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pluriSelect—讓陽選后的細胞與磁珠Say goodbye!
回顧上期（Cell Strainer-細胞過濾和純化的好幫手）pluriSelect公司不同孔徑規格的細胞篩網Mini Strainer和pluriStrainer深受大家的青睞。
小達君化身大白給細胞分選加些料，助力大家的分選實驗。今天介紹pluriSelect的一款分選的利器，不用千篇的內容就能掌握細胞分選的新思路， pluriSelect支招來搞定細胞陽選后的磁珠！
陽選后細胞攜帶的磁珠也可以去掉，這是個神馬操作呢？這么好的idea，全血分選當然不在話下，并且分選后血小板或紅細胞的污染非常低，得到的細胞也可以用于下游另一種細胞的陽性或陰性富集實驗。可分選的樣本類型也包括 PBMC, Buffy Coat, Cord Blood, Bone Marrow,Primary Cell Solution.
分選原理如下：
人的分選的指標如下（規格均為2ml裝）:
|
貨號 |
產品名稱 |
|
19-00200-10 |
CD2 S-pluriBead? anti-hu, 2ml |
|
19-00300-10 |
CD3 S-pluriBead? anti-hu, 2ml |
|
19-00400-10 |
CD4 S-pluriBead? anti-hu, 2ml |
|
19-00500-10 |
CD5 S-pluriBead? anti-hu, 2ml |
|
19-00700-10 |
CD7 S-pluriBead? anti-hu, 2ml |
|
19-00800-10 |
CD8 S-pluriBead? anti-hu, 2ml |
|
19-00900-10 |
CD9 S-pluriBead? anti-hu, 2ml |
|
19-011a0-10 |
CD11a S-pluriBead? anti-hu, 2ml |
|
19-011b0-10 |
CD11b S-pluriBead? anti-hu, 2ml |
|
19-01400-10 |
CD14 S-pluriBead? anti-hu, 2ml |
|
19-01500-10 |
CD15 S-pluriBead? anti-hu, 2ml |
|
19-01600-10 |
CD16 S-pluriBead? anti-hu, 2ml |
|
19-01900-10 |
CD19 S-pluriBead? anti-hu, 2ml |
|
19-02100-10 |
CD21 S-pluriBead? anti-hu, 2ml |
|
19-03100-10 |
CD31 S-pluriBead? anti-hu, 2ml |
|
19-04300-10 |
CD43 S-pluriBead? anti-hu, 2ml |
|
19-04400-10 |
CD44 S-pluriBead? anti-hu, 2ml |
|
19-04500-10 |
CD45 S-pluriBead? anti-hu, 2ml |
|
19-04700-10 |
CD47 S-pluriBead? anti-hu, 2ml |
|
19-04300-10 |
CD43 S-pluriBead? anti-hu, 2ml |
|
19-04400-10 |
CD44 S-pluriBead? anti-hu, 2ml |
|
19-04400-20 |
CD44 M-pluriBead? anti-hu, 2ml |
|
19-04500-10 |
CD45 S-pluriBead? anti-hu, 2ml |
|
19-04700-10 |
CD47 S-pluriBead? anti-hu, 2ml |
|
19-05500-10 |
CD55 S-pluriBead? anti-hu, 2ml |
|
19-09500-10 |
CD95 S-pluriBead? anti-hu, 2ml |
|
19-11700-10 |
CD117 S-pluriBead? anti-hu, 2ml |
|
19-13800-10 |
CD138 S-pluriBead? anti-hu, 2ml |
|
19-19300-10 |
CD193 S-pluriBead? anti-hu, 2ml |
|
19-235a0-10 |
CD235a S-pluriBead? anti-hu, 2ml |
|
19-27100-10 |
CD271 S-pluriBead? anti-hu, 2ml |
大鼠、小鼠、豬、羊、牛、狗、馬、兔子的分選指標如下（規格均為2ml裝）：
|
貨號 |
產品名稱 |
|
22-00400-10 |
CD4 S-pluriBead? anti-rat, 2ml |
|
22-008b0-10 |
CD8b S-pluriBead? anti-rat, 2ml |
|
22-011b0-10 |
CD11b S-pluriBead? anti-rat, 2ml |
|
22-04400-10 |
CD44 S-pluriBead? anti-rat, 2ml |
|
22-04500-10 |
CD45 S-pluriBead? anti-rat, 2ml |
|
22-05500-10 |
CD55 S-pluriBead? anti-rat, 2ml |
|
22-TCRAB-10 |
Anti TCRAB S-pluriBead? anti-rat, 2ml |
|
23-011b0-10 |
CD11b S-pluriBead? anti-porcine, 2ml |
|
23-01400-10 |
CD14 S-pluriBead? anti-porcine, 2ml |
|
23-02100-10 |
CD21 S-pluriBead? anti-porcine, 2ml |
|
23-02900-10 |
CD29 S-pluriBead? anti-porcine, 2ml |
|
23-04400-10 |
CD44 S-pluriBead? anti-porcine, 2ml |
|
23-04700-10 |
CD47 S-pluriBead? anti-porcine, 2ml |
|
23-062L0-10 |
CD62L S-pluriBead? anti-porcine, 2ml |
|
23-09000-10 |
CD90 S-pluriBead? anti-porcine, 2ml |
|
23-HLARP-10 |
HLARP S-pluriBead? anti-porcine, 2ml |
|
23-RBC00-10 |
RBC S-pluriBead? anti-porcine, 2ml |
|
24-02900-10 |
CD29 S-pluriBead? anti-sheep, 2ml |
|
24-04100-10 |
CD41/61 S-pluriBead? anti-sheep, 2ml |
|
24-04400-10 |
CD44 S-pluriBead? anti-sheep, 2ml |
|
24-04500-10 |
CD45 S-pluriBead? anti-sheep, 2ml |
|
24-04700-10 |
CD47 S-pluriBead? anti-sheep, 2ml |
|
24-062L0-10 |
CD62L S-pluriBead? anti-sheep, 2ml |
|
24-27100-10 |
CD271 S-pluriBead? anti-sheep, 2ml |
|
24-HLARP-10 |
HLARP S-pluriBead? anti-sheep, 2ml |
|
25-00900-10 |
CD9 S-pluriBead? anti-bovine, 2ml |
|
25-01400-10 |
CD14 S-pluriBead? anti-bovine, 2ml |
|
25-02100-10 |
CD21 S-pluriBead? anti-bovine, 2ml |
|
25-045R0-10 |
CD45R S-pluriBead? anti-bovine, 2ml |
|
25-04700-10 |
CD47 S-pluriBead? anti-bovine, 2ml |
|
25-062L0-10 |
CD62L S-pluriBead? anti-bovine, 2ml |
|
25-11700-10 |
CD117 S-pluriBead? anti-bovine, 2ml |
|
25-HLARP-10 |
HLARP S-pluriBead? anti-bovine, 2ml |
|
27-00400-10 |
CD4 S-pluriBead? anti-canine, 2ml |
|
27-02900-10 |
CD29 S-pluriBead? anti-canine, 2ml |
|
29-00300-10 |
CD3 S-pluriBead? anti-ms, 2ml |
|
29-00400-10 |
CD4 S-pluriBead? anti-ms, 2ml |
|
29-00800-10 |
CD8 S-pluriBead? anti-ms, 2ml |
|
29-011b0-10 |
CD11b S-pluriBead? anti-ms, 2ml |
|
29-011c0-10 |
CD11c S-pluriBead? anti-ms, 2ml |
|
29-01400-10 |
CD14 S-pluriBead? anti-ms, 2ml |
|
29-01900-10 |
CD19 S-pluriBead? anti-ms, 2ml |
|
29-03100-10 |
CD31 S-pluriBead? anti-ms, 2ml |
|
29-04500-10 |
CD45 S-pluriBead? anti-ms, 2ml |
|
29-32600-10 |
CD326 S-pluriBead? anti-ms, 2ml |
|
29-F4800-10 |
F4/80 S-pluriBead? anti-ms, 2ml |
|
29-Ly6G0-10 |
Ly6G S-pluriBead? anti-ms, 2ml |
|
31-GTaHS-10 |
universal S-pluriBead? anti-hamster, 2ml |
|
31-GTaMS-10 |
universal S-pluriBead? anti-ms, 2ml |
|
31-GTaRB-10 |
universal S-pluriBead? anti-rabbit, 2ml |
|
31-GTaRT-10 |
universal S-pluriBead? anti-rat, 2ml |
|
31-RBaGT-10 |
universal S-pluriBead? anti-goat, 2ml |
|
31-RBaHU-10 |
universal S-pluriBead? anti-hu, 2ml |
|
32-011a0-10 |
CD11a S-pluriBead? anti-horse, 2ml |
|
32-04100-10 |
CD41/61 S-pluriBead? anti-horse, 2ml |
|
33-01400-10 |
CD14 S-pluriBead? anti-rabbit, 2ml |
|
33-08100-10 |
CD81 S-pluriBead? anti-rabbit, 2ml |
不同來源的樣本的分選體系：
|
樣本類型 |
分選體系 |
|
Whole Blood |
40 μl S-pluriBead? suspension per 1 ml |
|
Other samples |
40 μl S-pluriBead? suspension per 1x106 targets |
|
Alternatively |
40 μl S-pluriBead? suspension per 1x106 total cells |
完成分選實驗需要搭配如下的輔助試劑：
|
貨號 |
產品名稱 |
|
50-01010-80 |
pluriPlix（用于樣本混勻孵育） |
|
70-50010-11 |
S-pluriBead? Mini Reagent Kit（Kit里的各成分可單獨訂購） |
|
70-50010-12 |
S-pluriBead? Maxi Reagent Kit（Kit里的各成分可單獨訂購） |
有木有發現自己的實驗中需要分選的指標呢？是不是對該分選方案充滿好奇呢，心動不如行動，那就聯系我們下單吧！
下一期我們會推出更精彩的內容，敬請期待！
*具體實驗步驟請點擊：”步驟“
